growth curve

Measurement of Growth Name : LEVENT Surname : DEMiRAY Student ID# : 140104012 surgical incision : molecular BIOLOGY AND GENETICS Date of Experiment : 27.02.2008 crimp In the first science testing groundoratory section of semester, melodic theme was measurement of microbial appendage. At the beginning, protester gave brief to the highest degree six measurement way of microbial growth and cast of microbial growth. The most commonly use measurement techniques atomic number 18 serial dilution and colony counting, microscopic mobile phone counts, membrane filtration, turbidity, mass determination and metabolic activity. In this lab cardinal of them serial dilution and colony counting and turbidity were employ only. In serial dilution and colony counting which is also named as a viable cell counts, concentrated samples are dilute by serial dilution and then diluted samples are plated on lively complete media. There are two metal plating methods, spread plating and pour plating. They both have some pros and cons. Moreover turbidity is based on the diffraction or dispense of light by bacteria. To measure absorbance rate spectrophotometer was used. The purpose of this view is learning measurement methods of microbial growth completely and practicing these techniques efficiently. Besides, plotting the represent of growth curve is one of the other purposes of this experiment.

Introduction In the laboratory, under convenient circumstances, a growing bacterial human beings doubles at regular! intervals. Growth is by geometric progression: 1, 2, 4, 8, etc. or 20, 21, 22, 23.........2n (where n = the number of generations). This is called exponential function growth. In fact, exponential growth is only vocalization of the bacterial life cycle, and not characteristic of the normal sample of growth of bacteria in nature (1). (2). Figure-1 As it can buoy be seen from the figure 1 the population of bacteria in a...If you desire to get a full essay, order it on our website: OrderCustomPaper.com

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